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KMID : 0848120120370030146
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International Journal of Oral Biology
2012 Volume.37 No. 3 p.146 ~ p.152
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MicroRNA Analysis during Cultured Odontoblast Differentiation
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Kim Do-Kyung
Park Min-Gyeong
Lee Myoung-Hwa
Yu Sun-Kyoung
Park Eu-Teum
Kim Seog
Lee Seul-Ah
Moon Yeon-Hee
Kim Heung-Joong
Kim Chun-Sung
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Abstract
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MicroRNAs (miRNAs, miRs) are about 21-25 nucleotides inlength and regulate mRNA translation by base pairing topartially complementary sites, predominantly in the 3’-untranslatedregion (3’-UTR) of the target mRNA. In this study, theexpression profile of miRNAs was compared and analyzed forthe establishment of miRNA-related odontoblast differentiationusing MDPC-23 cells derived from mouse dental papilla cells.To determine the expression profile of miRNAs during the differentiationof MDPC-23 cells, we employed miRNA microarrayanalysis, quantitative real-time PCR (qRT-PCR) andAlizaline red-S staining. In the miRNA microarray analysis, 11miRNAs were found to be up- or down-regulated more than3-fold between day 0 (control) and day 5 of MDPC-23 celldifferentiation among the 1,769 miRNAs examined. InqRT-PCR analysis, the expression levels of two of these molecules,miR-194 and miR-126, were increased and decreased inthe control MDPC-23 cells compared with the MDPC-23 cellsat day 5 of differentiation, respectively. Importantly, the overexpressionof miR-194 significantly accelerated mineralizationcompared with the control cultures during the differentiation ofMDPC-23 cells. These results suggest that the miR-194 augmentsMDPC-23 cell differentiation, and potently acceleratesthe mineralization process. Moreover, these in vitro results showthat different miRNAs are deregulated during the differentiationof MDPC-23 cells, suggesting the involvement of these genes inthe differentiation and mineralization of odontoblasts.
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KEYWORD
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miRNA, odontoblast, differentiation, microarray
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